Thermo Fisher Scientific CXCL12 beta (SDF-1 beta) Polyclonal Antibody, Biotin, PeproTech
Biotin으로 표지된 CXCL12 beta (SDF-1 beta) 폴리클로날 항체로, 인간 시료에 반응합니다. Western blot, ELISA, Immunostaining에 적합하며, 고순도 E.coli 유래 재조합 단백질로 면역화된 염소에서 생산되었습니다. 항원 친화 크로마토그래피로 정제된 연구용 시약입니다.
- 카탈로그번호
- 500-P87BGBT-xxxx (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CXCL12 beta (SDF-1 beta) Polyclonal Antibody, Biotin, PeproTech
Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.25–1.0 µg/mL |
| Immunostaining (IS) | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host/Isotype | Goat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived, 7.9 kDa Recombinant Human SDF-1beta (CXCL12) |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930831 |
Product Specific Information
AA Sequence of recombinant protein:
KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNKRF KM
Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human SDF-1beta (CXCL12). Anti-Human SDF-1beta (CXCL12)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
To detect Human SDF-1beta by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human SDF-1beta (500-P87BG) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human SDF-1beta.
Western Blot:
To detect Human SDF-1beta by Western Blot analysis, use at 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human SDF-1beta is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Packaging:
500-P87BGBT-1MG will be provided as 2 × 500 µg.
Target Information
CXCL12 is a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in metastasis of cancers such as breast cancer. Mutations in this gene are associated with resistance to HIV-1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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