Thermo Fisher Scientific CXCL12 alpha (SDF-1 alpha) Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific CXCL12 alpha (SDF-1 alpha) Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilutions

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human SDF-1alpha (CXCL12). Anti-Human SDF-1alpha (CXCL12)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
For detection of Human SDF-1alpha by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human SDF-1alpha (500-P87A) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human SDF-1alpha.

Western Blot:
For detection of Human SDF-1alpha by Western Blot, use at 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human SDF-1alpha is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Packaging:
500-P87ABT-1MG supplied as 2 × 500 µg.

Target Information

CXCL12 is a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in the metastasis of cancers such as breast cancer. Mutations in this gene are associated with resistance to HIV-1 infections. Multiple transcript variants encoding different isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.