Thermo Fisher Scientific IL17D Polyclonal Antibody, PeproTech
Human IL-17D 단백질을 인식하는 토끼 폴리클로날 항체로, Western Blot 및 ELISA에 최적화됨. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 감도를 제공. 연구용으로만 사용 가능.
- 카탈로그번호
- 500-P88-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IL17D Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.5–2.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human IL-17D |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930833 |
Product Specific Information
AA Sequence of recombinant protein:
APRAGRRPAR PRGCADRPEE LLEQLYGRLA AGVLSAFHHT LQLGPREQAR NASCPAGGRP ADRRFRPPTN LRSVSPWAYR ISYDPARYPR YLPEAYCLCR GCLTGLFGEE DVRFRSAPVY MPTVVLRRTP ACAGGRSVYT EAYVTIPVGC TCVPEPEKDA DSINSSIDKQ GAKLLLGPND APAGP.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IL-17D. Anti-Human IL-17D-specific antibody was purified by affinity chromatography employing an immobilized Human IL-17D matrix.
Sandwich ELISA:
To detect Human IL-17D by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-17D (500-P88Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human IL-17D.
Western Blot:
To detect Human IL-17D by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IL-17D is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
The protein encoded by this gene is a cytokine that shares sequence similarity with IL17. Treatment of endothelial cells with this cytokine stimulates production of other cytokines including IL6, IL8, and CSF2/GM-CSF. The increased expression of IL8 induced by this cytokine is NF-kappa B-dependent.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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