Thermo Fisher Scientific Phospho-PDHA1 (Ser293) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:1,000

ELISA

• Tested Dilution: 1 µg/mL

Product Specifications

Product Specific Information

• Positive test controls: 293T, NIH/3T3
• Target location: Mitochondrion matrix
• Immunogen sequence: GHSMS

Target Information

The pyruvate dehydrogenase (PDH) complex is a mitochondrial multienzyme complex that catalyzes the conversion of pyruvate to acetyl-CoA and CO₂, linking glycolysis to the TCA cycle.
It consists of three enzymatic components:

• Pyruvate dehydrogenase (E1)
• Dihydrolipoamide acetyltransferase (E2)
• Lipoamide dehydrogenase (E3)

The E1 enzyme is a heterotetramer of two alpha and two beta subunits. The PDHA1 gene encodes the E1 alpha 1 subunit, which contains the active site and is essential for PDH complex function.
Mutations in PDHA1 are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome.
Alternatively spliced transcript variants encoding different isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.