Thermo Fisher Scientific Phospho-p53 (Thr81) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:100–1:500

ELISA

• Tested Dilution: 1 µg/mL

Product Specifications

Product Specific Information

• Positive test controls include: C6
• Target locations: Cytoplasm, Endoplasmic reticulum, Mitochondrion matrix, Nucleus, PML body
• Immunogen sequence: APTPA

Target Information

The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or nucleotide starvation.
p53 regulates genes such as p21waf1 involved in G1 or G2/M arrest and is composed of:

• N-terminal transactivation domain
• Central DNA-binding domain
• Oligomerisation domain
• C-terminal regulatory domain

Phosphorylation at Ser15 is crucial for activation and stabilization, while Ser392 phosphorylation is associated with tumor formation.
p53 plays a role in preventing proliferation of damaged cells, acts as an anticancer agent, and is linked to aging and oxidative stress.
Mutations in p53 lead to loss of tumor suppressor activity and are found in many cancers, including Li-Fraumeni syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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