Thermo Fisher Scientific Phospho-Beclin 1 (Ser15) Polyclonal Antibody
Beclin 1 단백질의 Ser15 인산화 부위를 인식하는 Rabbit Polyclonal 항체. Western blot과 ELISA에 적합하며, Human, Mouse, Rat 시료에서 반응. 자가포식 관련 연구에 유용하며, 액상 형태로 -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:100–1:500 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic phosphorylated peptide around S15 of human Beclin 1 (NP_003757.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.93 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914929 |
Product Specific Information
Positive test controls include NIH/3T3, C6.
The target is usually found in the following locations: Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus, Mitochondrion, Mitochondrion membrane, Nucleus, Peripheral membrane protein, autophagosome, trans-Golgi network membrane.
Immunogen sequence: QVSFV
Target Information
Beclin 1 plays a central role in autophagy. It acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate.
Different PI3K complex forms are believed to play a role in multiple membrane trafficking pathways:
- PI3KC3-C1 is involved in the initiation of autophagosomes.
- PI3KC3-C2 is involved in the maturation of autophagosomes and endocytosis.
Beclin 1 also contributes to antiviral host defense.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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