Thermo Fisher Scientific M-CSF Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific M-CSF Polyclonal Antibody, Biotin (PeproTech)

Applications and Tested Dilution

• Western Blot (WB): 0.1–0.2 µg/mL
• ELISA: 0.25–1.0 µg/mL

Product Specifications

Product Specific Information

• AA Sequence of recombinant protein:
  MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP

• Preparation:
  Produced from sera of goats immunized with highly pure Recombinant Murine M-CSF. Anti-Murine M-CSF-specific antibody was purified by affinity chromatography and then biotinylated.

• Sandwich ELISA:
  To detect mM-CSF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine M-CSF (500-P62G) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant mM-CSF.

• Western Blot:
  To detect mM-CSF by Western Blot analysis, use at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant mM-CSF is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

• Packaging:
  500-P62GBT-1MG will be provided as 2 × 500 µg.

Target Information

M-CSF (Macrophage colony-stimulating factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. It also induces VEGF secretion by macrophages, mediating mobilization of endothelial progenitor cells and neovascularization. M-CSF exists as several bioactive isoforms differing in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. It is a disulfide-bonded homodimer processed into glycoprotein or proteoglycan isoforms. Binding to its receptor c-Fms (CSF-1R/CD115) induces receptor dimerization, internalization, and degradation. Functionally, M-CSF stimulates differentiation of hematopoietic stem cells to monocyte-macrophage populations. Human M-CSF shows activity on mouse cells, but mouse CSF does not act on human cells.

For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.

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