Thermo Fisher Scientific CD301b (MGL2) Monoclonal Antibody (11A10-B7), eBioscience

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

10 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

10 µg/mL

-

Flow Cytometry (Flow)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Not Applicable

Host/Isotype

Rat / IgG2a, lambda

Class

Monoclonal

Type

Antibody

Clone

11A10-B7

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• eFluor 660
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2865051

Product Specific Information

Description: The monoclonal antibody 11A10-B7 recognizes mouse CD301b. CD301b is also known as macrophage galactose-type C-type lectin 2 (MGL2). CD301b expression is restricted to dermal dendritic cells, connection tissue, and bone marrow derived dendritic cells. Macrophages also express CD301b at low levels. CD301b contains a single carbohydrate recognition domain that recognizes protein antigens that have a galactose and N-acetylgalactosamine (Gal/GalNAc). Gal/GalNAc protein antigens are efficiently internalized through CD301b and presented to antigen specific CD4+ cells via MHC Class II. CD301b+ dendritic cells are believed to play an important role in TH2 priming.

Applications Reported: This 11A10-B7 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, and microscopy.

Applications Tested: This 11A10-B7 antibody has been tested by immunohistochemistry of frozen mouse tissue and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD301b is also known as macrophage galactose-type C-type lectin 2 (MGL2). CD301b expression is restricted to dermal dendritic cells, connection tissue, and bone marrow derived dendritic cells. Macrophages also express CD301b at low levels. CD301b contains a single carbohydrate recognition domain that recognizes protein antigens that have a galactose and N-acetylgalactosamine (Gal/GalNAc). Gal/GalNAc protein antigens are efficiently internalized through CD301b and presented to antigen specific CD4+ cells via MHC Class II. CD301b+ dendritic cells are believed to play an important role in TH2 priming.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.