Thermo Fisher Scientific Human EGF Recombinant Protein

Applications

Product Specifications

Product Specific Information

Carrier-Free

• Reconstitution:
  Centrifuge the vial briefly before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile PBS buffer to a concentration of 0.1–1.0 mg/mL. Make any further dilutions in low endotoxin medium or a buffered solution containing a carrier protein such as heat-inactivated FCS or tissue culture-grade BSA.

• Storage:
  Store the lyophilized protein at 2°C to 8°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at ≤ -20°C. Avoid repeated freeze-thaw cycles.

Target Information

Epidermal Growth Factor (EGF) binds to the EGF receptor (EGFR), a 170 kDa protein kinase, initiating diverse cellular pathways in response to environmental stimuli. Upon EGF binding, EGFR forms homo- or heterodimers with other family members, triggering distinct signaling cascades via SH2 domain-containing effector proteins.

EGF is widely expressed in kidney, cerebrum, prostate, and salivary glands, acting as a potent mitogenic factor. The phosphorylated receptor recruits adapter proteins such as GRB2, activating downstream signaling pathways including RAS-RAF-MEK-ERK, PI3K-AKT, PLCγ-PKC, and STAT. It may also play a role in activating the NF-κB signaling cascade. Mutations in the EGF gene are linked to hypomagnesemia type 4 and have been associated with cancer progression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.