Thermo Fisher Scientific Human EGF Recombinant Protein
Recombinant Human EGF 단백질로, 세포 증식 및 신호전달 연구용으로 적합합니다. E. coli 발현, >95% 순도, 낮은 엔도톡신 수준(<0.1 ng/µg)을 특징으로 합니다. Lyophilized 형태로 제공되며, 다양한 기능적 및 면역학적 분석에 활용 가능합니다.
- 카탈로그번호
- PHG031x (4개 옵션)
- 카테고리
- Protein
- 판매단위
- pk
카탈로그
4개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Human EGF Recombinant Protein
Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | - |
| Immunohistochemistry (IHC) | Assay-dependent | - |
| ELISA | Assay-dependent | - |
| Functional Assay | Assay-dependent | View 5 publications |
| Control (Ctrl) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Published Species | Human |
| Expression System | E. coli |
| Molecular Weight | 6.2 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >95% by SDS-PAGE |
| Endotoxin Concentration | <0.1 ng/µg |
| Activity | ED50 < 0.4 ng/mL; determined by the dose-dependent proliferation of mouse BALB/3T3 cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
Carrier-Free
Reconstitution:
Centrifuge the vial briefly before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile PBS buffer to a concentration of 0.1–1.0 mg/mL. Make any further dilutions in low endotoxin medium or a buffered solution containing a carrier protein such as heat-inactivated FCS or tissue culture-grade BSA.Storage:
Store the lyophilized protein at 2°C to 8°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at ≤ -20°C. Avoid repeated freeze-thaw cycles.
Target Information
Epidermal Growth Factor (EGF) binds to the EGF receptor (EGFR), a 170 kDa protein kinase, initiating diverse cellular pathways in response to environmental stimuli. Upon EGF binding, EGFR forms homo- or heterodimers with other family members, triggering distinct signaling cascades via SH2 domain-containing effector proteins.
EGF is widely expressed in kidney, cerebrum, prostate, and salivary glands, acting as a potent mitogenic factor. The phosphorylated receptor recruits adapter proteins such as GRB2, activating downstream signaling pathways including RAS-RAF-MEK-ERK, PI3K-AKT, PLCγ-PKC, and STAT. It may also play a role in activating the NF-κB signaling cascade. Mutations in the EGF gene are linked to hypomagnesemia type 4 and have been associated with cancer progression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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