Thermo Fisher Scientific Human Heat Stable bFGF Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Functional Assay (Functional)

Assay-dependent

View 16 publications 16 publications

Control (Ctrl)

Assay-dependent

-

Product Specifications

Species

Human

Published species

Bovine, Human, Mouse

Expression System

E. coli

Amino acid sequence

155 aa + 20 aa for N-terminal tag for purification purposes

Molecular weight

19 kDa

Class

Recombinant

Type

Protein

Purity

>95% by SDS-PAGE

Endotoxin concentration

<1 EU/µg

Activity

ED50 = 0.6 - 1.1 ng/mL; determined by the dose-dependent proliferation of mouse NIH/3T3 fibroblast cells.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

20mM potassium phosphate with 750mM NaCl

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Wet ice

Product Specific Information

Carrier-Free

Reconstitution: Centrifuge the vial briefly, before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile, distilled water to a concentration of 1.0 mg/mL. Alternatively, reconstitute at lower concentrations in carrier-containing medium. Do not vortex. Apportion the reconstituted protein into working aliquots and store at -20°C. Make any further dilutions of the reconstituted protein in medium, or buffered solution containing carrier protein, such as PBS with 0.1% BSA. For the 5 µg unit size, reconstitute directly in carrier-containing medium. For other unit sizes, reconstitute to 1.0 mg/mL as follows:
• 50 µg, reconstitute in 50 µL
• 100 µg, reconstitute in 100 µL
• 500 µg, reconstitute in 500 µL

Storage: Store the lyophilized protein at -20°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at -20°C (not in a frost-free freezer) for up to 12 months. Avoid repeated freeze-thaw cycles.

Target Information

FGF2 (FGFb, fibroblast growth factor basic) belongs to the fibroblast growth factor (FGF) family, and interacts with high-affinity transmembrane receptors to influence cell proliferation and tissue neovascularization. FGF2 exists as five isoforms with distinct intracellular localizations and functions. The 18 kDa isoform is predominantly cytosolic and acts through cell surface receptors, whereas the 22, 22.5, 24 and 34 kDa isoforms are nuclear and may signal independent of transmembrane receptor pathways. In humans, the gene is located on the q arm of chromosome 4. FGF2 has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for FGF2 contains multiple polyadenylation sites, and is alternatively translated from non-AUG and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. Diseases associated with FGF2 dysfunction include Kaposi Sarcoma and corneal neovascularization.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.