Thermo Fisher Scientific Human Heat Stable bFGF Recombinant Protein
인간 유래 열안정성 bFGF 재조합 단백질로, 세포 증식 및 혈관신생 연구에 적합합니다. E. coli 발현, >95% 순도, ED50 0.6–1.1 ng/mL의 높은 생물활성을 보입니다. 동결건조 형태로 제공되며, 재구성 후 -20°C에서 장기 보관 가능합니다.
- 카탈로그번호
- PHG036x (4개 옵션)
- 카테고리
- Protein
- 판매단위
- pk
카탈로그
4개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Human Heat Stable bFGF Recombinant Protein
Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA: Assay-dependent
- Functional Assay: Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Published species | Bovine, Human, Mouse |
| Expression System | E. coli |
| Amino Acid Sequence | 155 aa + 20 aa (N-terminal tag for purification) |
| Molecular Weight | 19 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >95% by SDS-PAGE |
| Endotoxin Concentration | <1 EU/µg |
| Activity | ED50 = 0.6–1.1 ng/mL (NIH/3T3 proliferation assay) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Storage Buffer | 20 mM potassium phosphate, 750 mM NaCl |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
Product Specific Information
Carrier-Free
Reconstitution:
- Briefly centrifuge vial before opening.
- Reconstitute lyophilized protein in sterile distilled water to 1.0 mg/mL.
- Alternatively, reconstitute in carrier-containing medium.
- Do not vortex.
- Prepare working aliquots and store at -20°C.
- Dilute further in medium or buffer containing carrier protein (e.g., PBS with 0.1% BSA).
Reconstitution volumes:
- 50 µg → 50 µL
- 100 µg → 100 µL
- 500 µg → 500 µL
Storage:
- Store lyophilized protein at -20°C, preferably desiccated.
- After reconstitution, aliquot and store at -20°C (not frost-free freezer) for up to 12 months.
- Avoid repeated freeze-thaw cycles.
Target Information
FGF2 (basic fibroblast growth factor, bFGF) is a member of the fibroblast growth factor family that binds high-affinity transmembrane receptors to regulate cell proliferation and neovascularization.
It exists as five isoforms with distinct intracellular localizations and functions:
- The 18 kDa isoform is cytosolic and acts via surface receptors.
- The 22, 22.5, 24, and 34 kDa isoforms are nuclear and may signal independently of receptor pathways.
In humans, the FGF2 gene is located on chromosome 4q.
It plays roles in limb and nervous system development, wound healing, and tumor growth.
FGF2 mRNA has multiple polyadenylation sites and is alternatively translated from AUG and non-AUG codons, producing isoforms with distinct properties.
CUG-initiated isoforms mediate intracrine effects (nuclear), while AUG-initiated forms mediate paracrine and autocrine effects (cytosolic).
FGF2 dysfunction is associated with diseases such as Kaposi Sarcoma and corneal neovascularization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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