Thermo Fisher Scientific IL-22 Monoclonal Antibody (22URTI), FITC, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested dilution: 5 µL (0.125 µg)/test
  View 10 publications

Product Specifications

Additional Formats Available

• Brilliant UV 395
• Brilliant UV 563
• Brilliant UV 661
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 605
• eFluor 450
• eFluor 660
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

Description:
The 22URTI monoclonal antibody reacts with human interleukin (IL)-22, a 20 kDa member of the IL-10 cytokine family secreted primarily by Th17 and NK cells. Other T cells can also produce IL-22. In Th17 cultures, IL-22 expression is induced more by IL-23 than IL-6 or TGF-β, suggesting secretion by more differentiated Th17 cells in vivo. The IL-22 receptor is a heterodimer of IL-10R2 and IL-22R1.

Applications Reported:
This antibody is suitable for intracellular staining followed by flow cytometric analysis.

Applications Tested:
Pre-titrated and validated for intracellular staining of restimulated, Th17-polarized CD4⁺ human PBMCs using the Intracellular Fixation and Permeabilization Buffer Set (Product #88-8824-00) and the BestProtocols® protocol (Protocol A: Two-step protocol for cytoplasmic proteins).
Recommended use: 5 µL (0.125 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Fluorescence Characteristics:

• Excitation: 488 nm
• Emission: 520 nm
• Laser: Blue Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

IL-22, also known as IL-10-related T-cell derived inducible factor, is an α-helical cytokine in the IFN-IL-10 family (including IL-19, IL-20, IL-24, IL-26, IL-28, IL-29). It is mainly produced by activated T cells and NK cells. The IL-22 gene is located on chromosome 12q and structurally related to IL-10. IL-22 acts via a heterodimeric receptor complex (IL-22R1 and IL-10R2), activating the JAK-STAT pathway.
A soluble receptor, IL-22BP, antagonizes IL-22 binding and signaling. IL-22 primarily affects nonimmune cells such as epithelial cells, keratinocytes, and hepatocytes, regulating genes linked to innate immunity, wound healing, and inflammation. It is co-expressed with IL-17A/F in Th17 cells but differentially regulated. IL-22 plays a critical role in host defense against extracellular pathogens and promotes tissue repair.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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