Thermo Fisher Scientific IL-1 beta (Pro-form) Monoclonal Antibody (NJTEN3), FITC, eBioscience
마우스 IL-1β 전구체(pro-form)에 특이적인 단클론 항체로, 염증 관련 세포 내 단백질 분석에 적합합니다. FITC 형광 표지로 유세포분석(Flow cytometry)에 사용 가능하며, 0.25 µg/test 권장. 4°C 어둠에서 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 2 |
| Immunocytochemistry (ICC/IF) | - | 1 |
| Flow Cytometry (Flow) | 0.25 µg/test | 23 |
| ELISA | - | 2 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | NJTEN3 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10718839 |
Additional Formats Available
- APC
- APC-eFluor 780
- eFluor 450
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
This NJTEN3 monoclonal antibody reacts with the pro-form of mouse IL-1 beta, a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. It recognizes only the pro-form (31 kDa) and not the active (17 kDa) cleaved form. Activation of inflammasomes triggers cleavage of pro-IL-1β via caspase-1 activation, leading to secretion through a non-classical pathway. IL-1β signals via IL-1RI, shared with IL-1α, and plays a key role in innate immune responses. Its activity can be inhibited by IL-1RA. Elevated IL-1β levels are linked to chronic inflammatory conditions.
Applications Reported: Intracellular staining and flow cytometric analysis.
Applications Tested: Tested using mouse thioglycolate-elicited peritoneal macrophages with the Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00). Recommended use ≤0.25 µg/test (100 µL final volume). Optimize antibody titration for best results.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Interleukin-1 beta (IL-1β) is a proinflammatory cytokine produced by monocytes, macrophages, and dendritic cells. Synthesized as a 31 kDa pro-form, it is cleaved into an active 17 kDa form via inflammasome-mediated caspase-1 activation. IL-1β lacks a signal peptide and is secreted through an alternative pathway. It signals through IL-1RI and IL-1RII, shared with IL-1α, inducing proinflammatory cytokines and acute-phase responses. IL-1RA competitively inhibits receptor binding. Elevated IL-1β is associated with chronic inflammation, making IL-1β and IL-1RA targets for therapeutic research.
For Research Use Only. Not for diagnostic use or resale without authorization.
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