Thermo Fisher Scientific IL-10 Monoclonal Antibody (JES5-16E3), FITC, eBioscience
마우스 IL-10을 인식하는 JES5-16E3 클론의 단클론 항체로, FITC 형광 표지되어 Flow Cytometry, ELISA, Neutralization 실험에 적합합니다. 0.5 µg/test로 사용되며, 488/520 nm 파장에서 검출됩니다. 연구용으로만 사용 가능합니다.
- 카탈로그번호
- 11-7101-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IL-10 Monoclonal Antibody (JES5-16E3), FITC, eBioscience
Thermo Fisher Scientific IL-10 Monoclonal Antibody (JES5-16E3), FITC, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry | 0.5 µg/test | View 34 publications |
| ELISA | - | View 1 publication |
| Neutralization | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | JES5-16E3 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465402 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 700
- APC
- Biotin
- Functional Grade
- PE
- PerCP-Cyanine5.5
- Request custom conjugation available
Product Specific Information
The JES5-16E3 antibody reacts with mouse interleukin-10 (IL-10), an ~18 kDa cytokine also known as Cytokine Synthesis Inhibitory Factor (CSIF). IL-10 is produced by Th2 cells, B1 cells, macrophages, and keratinocytes. It inhibits Th1 cytokine synthesis and T cell proliferation, while promoting mast cell activation and Th2 response.
Applications Reported:
Used for ELISA, ELISPOT, intracellular flow cytometry, IHC, and neutralization assays.
Applications Tested:
Validated for intracellular staining in cultured mouse splenocytes. Recommended usage ≤0.5 µg/test (100 µL final volume). Optimal titration should be determined empirically.
Optical Properties:
Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Post-manufacturing filtration: 0.2 µm
Target Information
IL-10 is a cytokine mainly produced by monocytes and lymphocytes. It regulates immune responses by down-regulating Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. It enhances B cell survival and antibody production, inhibits NF-κB activity, and modulates the JAK-STAT pathway. Mouse knockout studies confirm its role as a key immunoregulator in the intestinal tract. Mutations are linked to HIV-1 susceptibility and rheumatoid arthritis. Associated diseases include Graft-Versus-Host Disease and HIV-1 infection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.