Thermo Fisher Scientific p21WAF1 (Tumor Suppressor Protein) Recombinant Rabbit Monoclonal Antibody (CIP1/2275R)
p21WAF1 종양억제 단백질을 인식하는 재조합 토끼 단클론 항체로, 인간 시료에 반응하며 Mouse 및 Rat에는 반응하지 않음. Western blot과 면역조직화학(IHC)에 적합. p53 경로 연구 및 세포주기 조절 관련 연구에 활용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1–2 µg/mL
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: Assay-dependent
Immunohistochemistry (PFA fixed) (IHC (PFA))
- Tested Dilution: 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | CIP1/2275R |
| Immunogen | Recombinant full-length human CDKN1A protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Does not react with Mouse or Rat
Target Information
Protein p21 is a tumor suppressor protein that inhibits Cyclin-dependent kinases (CDK) and is transcriptionally activated by p53.
p21 plays a crucial role in the cellular response to genotoxic stress and acts as a major transcriptional target of p53.
In the nucleus, p21 binds and inhibits Cdk1 and Cdk2, blocking the transition from G1 to S phase or G2 to mitosis after DNA damage, allowing DNA repair.
In the cytoplasm, p21 has anti-apoptotic effects by inhibiting caspase 3 and apoptotic kinases ASK1 and JNK.
p21 exhibits dual roles in carcinogenesis — functioning as both a tumor suppressor and oncogene depending on context.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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