Thermo Fisher Scientific VISTA Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Immunocytochemistry (ICC/IF)

5-15 µg/mL

-

Flow Cytometry (Flow)

0.25 µg/10^6 cells

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Sheep / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse VISTA/B7-H5/PD-1H Phe33-Ala191 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:VISTA,uniProtId:Q9D659-1,ncbiNodeId:10090,antigenRange:33-191,antigenLength:308,antigenImageFileName:PA5-47032_VISTA_Q9D659-1_Sheep.svg,antigenImageFileNamePDP:PA5-47032_VISTA_Q9D659-1_Sheep_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2606501

Product Specific Information

In direct ELISAs, approximately 25% cross-reactivity with recombinant human VISTA/B7-H5/PD-1H is observed.

Reconstitute in sterile PBS to a final concentration of 0.2 mg/mL.

Target Information

VISTA (V-domain Ig containing suppressor of T-cell activation), also known as B7-H5 or PD-1H, is a product of the VSIR gene. Although VISTA bears sequence homology to PD-L1 it has a different expression pattern. In both mouse and humans, VISTA is expressed predominantly on hematopoietic cells with the highest densities on myeloid cells, and lower levels on T cells. It is usually absent on B cells. VISTA expressed by antigen presenting cells has been shown to directly suppress proliferation and cytokine production of CD4 and CD8 T cells, making it a target for cancer immunotherapy. The reported ligands of VISTA include CD28H, VSIG-3 and VSIG-8.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.