Thermo Fisher Scientific IL-17A Polyclonal Antibody, Biotin, PeproTech
Biotin으로 표지된 Rabbit Polyclonal Anti-Mouse IL-17A 항체로, ELISA 및 Western Blot에 적합합니다. 고순도 재조합 Murine IL-17A로 면역하여 제작되었으며, 항원 친화 크로마토그래피로 정제되었습니다. 염증 관련 연구 및 세포 신호 분석에 유용합니다.
- 카탈로그번호
- 500-P265BT-xxxx (2개 옵션)
- 판매단위
- pk
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Thermo Fisher Scientific IL-17A Polyclonal Antibody, Biotin, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.25–1.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Murine IL-17A |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930150 |
Product Specific Information
AA Sequence of recombinant protein:
AAIIPQSSAC PNTEAKDFLQ NVKVNLKVFN SLGAKVSSRR PSDYLNRSTS PWTLHRNEDP DRYPSVIWEA QCRHQRCVNA EGKLDHHMNS VLIQQEILVL KREPESCPFT FRVEKMLVGV GCTCVASIVR QAA.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-17A. Anti-Murine IL-17A-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
To detect mIL-17A by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine IL-17A (500-P265) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of recombinant mIL-17A.
Western Blot:
To detect mIL-17A by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for recombinant mIL-17A is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Packaging:
500-P265BT-1MG will be provided as 2 × 500 µg.
Target Information
Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection.
IL-17A is a 32 kDa disulfide-linked homodimer consisting of 136 amino acids, part of the IL-17A–F family, and signals through the IL-17 receptor (IL-17R/CDw217).
It enhances ICAM-1 expression in human fibroblasts and stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2.
IL-17A regulates NF-kappa B and MAP kinase activities, stimulates IL-6 and COX-2 expression, and enhances nitric oxide (NO) production.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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