Thermo Fisher Scientific APAF1 Polyclonal Antibody
APAF1 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체. Western blot 및 IHC에 최적화되어 있으며, 인간, 마우스, 랫트 시료에 반응. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성 제공. 냉장 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | View 2 publications |
| Immunohistochemistry (IHC) | 20 µg/mL | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A peptide corresponding to amino acids near the carboxy terminus of human Apaf1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long-term storage, store at -20°C. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11154347 |
Product Specific Information
- In Western blot applications, this antibody detects a band at approximately 130 kDa.
- Suggested positive control: human heart tissue lysate.
Target Information
APAF1 gene encodes a cytoplasmic protein that initiates apoptosis.
This protein contains multiple WD-40 domains, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC).
Upon binding cytochrome c and dATP, it forms an oligomeric apoptosome that activates caspase 9, triggering the caspase cascade leading to apoptosis.
Alternative splicing results in several transcript variants encoding different isoforms.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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