Thermo Fisher Scientific ATF2 Polyclonal Antibody
ATF2 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC(P/F), Flow cytometry에 적합합니다. Human, Mouse, Rat에 반응하며, 항원 친화 크로마토그래피로 정제되었습니다. 동결건조 형태로 제공되며, 재구성 시 500 µg/mL 농도로 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 0.5–1 µg/mL |
| Immunohistochemistry (Frozen) (IHC (F)) | 0.5–1 µg/mL |
| Flow Cytometry (Flow) | 1–3 µg/1×10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived human ATF2 recombinant protein (Position: E93–E450) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5 mg BSA |
| Contains | 0.05 mg sodium azide |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2745947 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and belongs to the ATF/CREB family of leucine zipper proteins.
ATF-2 interacts with various viral oncoproteins and cellular tumor suppressors and serves as a target of the SAPK/JNK and p38 MAP kinase signaling pathways.
Cellular stress such as genotoxic agents, inflammatory cytokines, and UV irradiation stimulates ATF-2 transcriptional activity.
Activation occurs via phosphorylation at Thr69 and Thr71, mediated by SAPK and p38 MAPK in vitro and in transfected cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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