
Thermo Fisher Scientific Phospho-eNOS (Ser1177) Polyclonal Antibody
Phospho-eNOS (Ser1177) 특이적 Rabbit Polyclonal Antibody로, 인산화된 eNOS 단백질 검출에 적합. Western blot, IHC, ELISA에 사용 가능. Human 및 Mouse 반응성. Affinity Chromatography로 정제된 고품질 항체로, 세포막 및 세포질 내 eNOS 인산화 상태 연구에 유용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | – |
| ELISA | 1 µg/mL | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic phosphorylated peptide around S1177 of human eNOS (NP_0005942) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.91 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2809173 |
Product Specific Information
- Positive Samples: SKOV3, NIH/3T3
- Cellular Location: Cell membrane, Cytoplasm, Golgi apparatus, Membrane, caveola, cytoskeleton
- Immunogen sequence: TQSFSL
Target Information
Nitric oxide (NO) is an inorganic, gaseous free radical that mediates various intercellular communications including vasorelaxation, neurotransmission, and cytotoxicity. NO production is catalyzed by nitric oxide synthase (NOS), which oxidizes L-arginine to produce L-citrulline and NO.
There are three NOS isoforms:
- Neuronal NOS (nNOS, type I)
- Endothelial NOS (eNOS, type III)
- Inducible NOS (iNOS, type II)
All NOS isoforms contain calmodulin, NADPH, FAD, and FMN binding domains.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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