Thermo Fisher Scientific OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal Antibody (eBio25-D1.16 (25-D1.16)), Brilliant Violet 711, eBioscience
Ovalbumin 유래 SIINFEKL 펩타이드가 결합된 H-2Kb 복합체를 인식하는 마우스 단클론 항체. Brilliant Violet 711 형광염료로 표지되어 유세포 분석에 최적. 마우스 면역세포 내 항원 제시 세포(APC) 추적에 유용. 광민감성이 있어 암실 보관 필요.
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Thermo Fisher Scientific OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal Antibody
Clone: eBio25-D1.16 (25-D1.16)
Conjugate: Brilliant Violet™ 711
Brand: eBioscience™
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.25 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Brilliant Violet™ 711 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio25-D1.16 (25-D1.16) |
| Conjugate | Brilliant Violet™ 711 |
| Excitation / Emission Max | 406 / 711 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_3074098 |
Available Formats
- Unconjugated
- APC
- Biotin
- Brilliant UV 395
- Brilliant UV 615
- Brilliant UV 737
- Brilliant Violet 650
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Custom conjugation available
Product Specific Information
The 25-D1.16 monoclonal antibody reacts with the ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I, but not with unbound H-2Kb or irrelevant peptide-bound forms.
It is useful for tracking the quantity and localization of specific antigen-presenting cells (APC) in vivo.
Applications Reported: Flow cytometric analysis
Applications Tested: Mouse splenocytes; ≤0.25 µg/test (100 µL final volume). Cell number may range from 10⁵–10⁸ cells/test. Optimal titration recommended.
Fluorochrome Information:
Brilliant Violet™ 711 (BV711) emits at 713 nm and is suitable for cytometers equipped with a violet (405 nm) laser. Verify instrument compatibility.
Staining Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.
Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect vial and stained samples from light.
Fixation:
Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.
BV711 is not compatible with methanol-based fixation.
Optical Properties:
- Excitation: 407 nm
- Emission: 713 nm
- Laser: Violet Laser
Trademark Notice:
BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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