Thermo Fisher Scientific Ki-67 Monoclonal Antibody (SolA15), Brilliant Violet 711, eBioscience

Thermo Fisher Scientific Ki-67 Monoclonal Antibody (SolA15), Brilliant Violet 711, eBioscience™

Applications

• Flow Cytometry (Flow): Tested at 1.0 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• Alexa Fluor Plus 594
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 786
• eFluor 450, 506, 570, 615, 660
• FITC
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein expressed during all active phases of the cell cycle (G1, S, G2, and mitosis), but absent in resting cells (G0). Ki-67 serves as a proliferation marker, indicating the growth fraction of a cell population. It is also detected in human, non-human primate, and canine Ki-67.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Tested using mouse splenocytes and the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00). Recommended ≤1.0 µg/test.

For optimal performance, antibody titration is recommended.
Brilliant Violet™ 711 (BV711) emits at 713 nm and is suitable for cytometers with a 405 nm violet laser.

When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (#SB-4401-42) or Brilliant Stain Buffer™ (#00-4409-75) to minimize non-specific interactions.

Light Sensitivity: Protect from light to prevent photo-induced oxidation.
Fixation: Compatible with IC Fixation Buffer (#00-8222-49) or 1-step Fix/Lyse Solution (#00-5333-54) for up to 3 days at 4°C.
Note: BV711 is not compatible with methanol-based fixation.
Excitation: 407 nm; Emission: 713 nm; Laser: Violet.

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

Ki-67 is a nuclear protein expressed during late G1, S, G2, and M phases of the cell cycle. It associates with euchromatin and heterochromatin through its FHA domain. Ki-67 expression correlates with cell proliferation and is absent in G0 cells. It is a key marker for assessing tumor growth and cell cycle progression, with strong correlation between low Ki-67 index and low-grade tumors. Commonly used as a neuronal proliferation marker.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.