Thermo Fisher Scientific FOXP3 Monoclonal Antibody (FJK-16s), Brilliant Violet 711, eBioscience

Thermo Fisher Scientific FOXP3 Monoclonal Antibody (FJK-16s), Brilliant Violet 711, eBioscience

Applications and Tested Dilution

• Flow Cytometry (Flow): 1.0 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC
• Biotin
• Brilliant Violet 421, 605, 786
• eFluor 450, 506, 570, 615, 660
• FITC
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Custom conjugation available

Product Specific Information

The FJK-16s antibody reacts with mouse, rat, dog, porcine, bovine, and cat FOXP3 (FORKHEAD BOX P3, SCURFIN, JM2). FOXP3 is a 49–55 kDa transcriptional regulator associated with CD4+CD25+ regulatory T cells (Treg cells).
Epitope mapped to amino acids 75–125 of mouse FOXP3. In humans, this region may be alternatively spliced.
Optimized for use with Foxp3/Transcription Factor Buffer Staining Set (Product #00-5523-00). Use of other buffers is not recommended.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Intracellular staining of mouse splenocytes using Foxp3/Transcription Factor Staining Buffer Set. Recommended ≤1.0 µg/test (100 µL final volume). Cell count range: 10⁵–10⁸ cells/test. Titrate for optimal performance.

Fluorochrome Information:
Brilliant Violet™ 711 (BV711) emits at 713 nm, excitable by 405 nm violet laser. Use appropriate cytometer settings.
For panels with multiple polymer dyes, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to reduce non-specific interactions.

Handling and Fixation:

• Light sensitive; protect from light.
• Compatible with IC Fixation Buffer or 1-step Fix/Lyse Solution (up to 3 days at 4°C).
• Not compatible with methanol-based fixation.
• Excitation: 407 nm; Emission: 713 nm; Laser: Violet.

Trademark Notice:
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

FOXP3 (Forkhead box protein 3) is a transcriptional regulator essential for immune homeostasis.

• Highly expressed in CD25+CD4+ regulatory T cells.
• Defects cause scurfy phenotype in mice and IPEX syndrome in humans.
• FOXP3 expression regulated by p53 in human cancer cells.
• Multiple transcript variants identified.

For Research Use Only. Not for diagnostic use or resale without authorization.

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