Thermo Fisher Scientific IgM Monoclonal Antibody (II/41), Brilliant Violet 711, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 1.0 µg/test

Product Specifications

Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant Violet 605
• eFluor 450
• eFluor 570
• eFluor 660
• FITC
• NovaFluor Red 710
• NovaFluor Yellow 610
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 780
• Request custom conjugation

Product Specific Information

The II/41 monoclonal antibody reacts with the µ heavy chain of mouse IgM and does not cross-react with IgD, IgG, or IgA. IgM is expressed intracellularly during early B cell development and on the surface of mature B cells. Fluorochrome-conjugated II/41 can be used as a detection secondary for mouse IgM.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse bone marrow cells at ≤1.0 µg/test (final volume 100 µL; 10⁵–10⁸ cells/test). Optimal titration recommended.
• Fluorochrome: Brilliant Violet™ 711 (BV711), emission at 713 nm, for use with violet (405 nm) laser.
• Buffer Recommendation: When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to reduce non-specific interactions.
• Light Sensitivity: Protect from light to prevent photo-induced oxidation.
• Fixation: Compatible with IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C. Not compatible with methanol-based fixation.
• Excitation: 407 nm
• Emission: 713 nm
• Laser: Violet Laser

Note: BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

Mouse IgM is an immunoglobulin produced by B cells as the first antibody during an immune response. It is a pentamer composed of five heavy and five light chains, involved in complement activation and antigen agglutination. Mouse IgM plays a key role in innate immunity and is widely used in ELISA, Western blotting, and immunohistochemistry research applications.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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