Thermo Fisher Scientific MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), Brilliant Violet 711, eBioscience
마우스 MHC Class II (I-A/I-E) 항원을 인식하는 단클론 항체로, Brilliant Violet 711 형광체가 결합된 제품입니다. Flow Cytometry용으로 검증되었으며, 높은 특이성과 안정성을 제공합니다. 4°C 암소 보관, 연구용 전용 시약입니다.
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Thermo Fisher Scientific MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), Brilliant Violet 711, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.25 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), Brilliant Violet™ 711, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M5/114.15.2 |
| Conjugate | Brilliant Violet™ 711 |
| Excitation / Emission Max | 406 / 711 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2937203 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488, 532, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 496, 661, 737
- Brilliant Violet 421, 605, 650, 786
- eFluor 450, 506
- FITC
- Functional Grade
- NovaFluor Blue/Red/Yellow series
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Custom conjugation available upon request
Product Specific Information
The M5/114.15.2 monoclonal antibody reacts with mouse MHC class II molecules (I-A and I-E subregion-encoded glycoproteins). It detects polymorphic determinants on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying specific H-2 haplotypes (H-2 b, d, q, p, r, u).
- Applications Reported: Flow cytometric analysis
- Applications Tested: Mouse splenocytes, ≤0.25 µg/test
- Recommended Use: Carefully titrate for optimal performance
- Laser Compatibility: Violet laser (405 nm)
- Emission Peak: 713 nm
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.
Light Sensitivity: Protect from light; tandem dye sensitive to photo-induced oxidation.
Fixation: Compatible with IC Fixation Buffer or 1-step Fix/Lyse Solution for up to 3 days at 4°C in dark.
Not Compatible: Methanol-based fixation.
Trademark Notice: BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.
Target Information
MHC class II molecules are transmembrane glycoproteins expressed on professional antigen-presenting cells (macrophages, dendritic cells, B cells). They present endocytosed exogenous antigens to T cells via a peptide-binding groove 15–24 amino acids long.
HLA and MHC antibodies are crucial in Immunopeptidomics, aiding neoantigen identification through LC-MS/MS analysis.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without authorization.
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