Thermo Fisher Scientific IL-4 Monoclonal Antibody (30340)
마우스 IL-4를 인식하는 Rat IgG1 단클론 항체로 Western blot, ELISA, 중화시험에 적합. 단백질 A/G로 정제된 동결건조 형태이며, 재구성 후 사용. 보존제 없이 PBS 및 5% 트레할로오스에 저장. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | - |
| ELISA (Detection) | 0.1–0.4 µg/mL | - |
| ELISA (Capture) | 2–8 µg/mL | - |
| Neutralization (Neu) | 0.1–0.6 µg/mL | View 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 30340 |
| Immunogen | E. coli-derived recombinant mouse IL-4 (His23–Ser140) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2609637 |
Product Specific Information
- Reconstitute at 0.5 mg/mL in sterile PBS.
- Endotoxin level: < 0.10 EU per 1 µg of antibody (LAL method).
Target Information
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells and serves as a ligand for the interleukin-4 receptor. IL-4 exhibits a broad range of biological activities. The IL-4 receptor also binds IL-13, contributing to overlapping functions between these cytokines. STAT6 acts as a key mediator in IL-4 signaling. The IL-4 gene, along with IL-3, IL-5, IL-13, and CSF2, forms a cytokine gene cluster on chromosome 5q. IL-4 is located near IL-13, and both genes, along with IL-5, are co-regulated by long-range elements spanning approximately 120 kb. IL-4 delta 2 is a known splice variant of IL-4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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