Thermo Fisher Scientific IFN gamma Monoclonal Antibody (25718)
Human IFN gamma를 인식하는 Mouse IgG2a 단일클론 항체로 다양한 면역분석(IHC, ICC/IF, Flow, Neutralization)에 사용 가능. Lyophilized 형태, Protein A/G 정제, PBS+5% trehalose 저장. 고순도, 낮은 endotoxin 수준(<0.10 EU/µg).
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 8–25 µg/mL | - |
| Flow Cytometry (Flow) | 0.25 µg/10^6 cells | - |
| Neutralization (Neu) | 0.02–0.06 µg/mL | - |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Published Species | Rhesus monkey |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 25718 |
| Immunogen | E. coli-derived recombinant human IFN-gamma (Gln24–Gln166) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2609805 |
Product Specific Information
- Reconstitute at 0.5 mg/mL in sterile PBS.
- Endotoxin level: <0.10 EU per 1 µg of antibody (LAL method).
Target Information
IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, and other cytokines.
It is a dimeric glycoprotein composed of two 146 amino acid subunits (~45 kDa functional homodimer).
On SDS-PAGE, IFN gamma shows 25, 20, and minor 15.5 kDa bands due to differential glycosylation.
Human IFN gamma is highly species specific and does not cross-react with mouse IFN gamma.
Functions include:
- Antiviral and tumor antiproliferative activity
- Induction of class I and II MHC
- Macrophage activation
- Enhanced immunoglobulin secretion by B lymphocytes
- Cytokine regulation and synergy with other cytokines
Activation occurs via IFN gamma receptor I/II and the JAK-STAT pathway.
Human IFN gamma shares ~40% sequence homology with mouse IFN gamma.
Upregulated by IL2, FGF basic, EGF; downregulated by vitamin D3 or DMN.
Mutations in the IFN gamma gene are associated with aplastic anemia.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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