Thermo Fisher Scientific AKT3 Monoclonal Antibody (9A5.H9.G7), PE
AKT3 단백질을 인식하는 Thermo Fisher Scientific의 PE-conjugated mouse monoclonal antibody입니다. Western blot, IHC, Flow cytometry 등 다양한 응용에 적합하며, Human, Mouse, Rat에서 반응합니다. 단백질 A로 정제되었으며, 형광 특성은 565/576 nm입니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000 | |
| Immunohistochemistry (IHC) | Assay-dependent | |
| Flow Cytometry (Flow) | Assay-dependent | |
| ELISA | Assay-dependent | |
| Immunoprecipitation (IP) | Assay-dependent | |
| Dot Blot (DB) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 9A5.H9.G7 |
| Immunogen | Anti-AKT3 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT3 protein. |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.5M NaCl |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Available Formats
- Unconjugated
- APC
- Biotin
- FITC
- Custom conjugation available upon request
Product Specific Information
- Store vial at 4°C prior to restoration.
- Restore with deionized water (or equivalent).
- Stable at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
- Centrifuge product if not completely clear after standing at room temperature.
- Do not freeze after reconstitution.
- Store reagent in the dark and handle under subdued lighting.
Anti-AKT3 antibody is directed against human AKT3 and detects both unphosphorylated and phosphorylated forms of the protein.
Cross-reactivity with AKT3 from other species has not been determined, but the immunogen sequence shows 100% identity to human, mouse, and rat, suggesting expected cross-reactivity.
Cross-reactivity with AKT2 and AKT has not been determined.
Target Information
AKT3 (PKB gamma) is stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1).
It is involved in multiple biological processes including:
- Cell proliferation and differentiation
- Apoptosis and tumorigenesis
- Glycogen synthesis and glucose uptake
Alternative splice variants encoding distinct isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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