Thermo Fisher Scientific ERK2 Internal Monoclonal Antibody (3H9.A1.A8), Biotin
ERK2 단백질을 특이적으로 인식하는 Biotin 결합 단일클론 항체. Western blot과 ELISA에 최적화되어 있으며, Human, Mouse, Rat 시료에 반응. Protein A로 정제된 고순도 항체로, 안정적 장기 보관 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| ELISA | 1:40,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3H9.A1.A8 |
| Immunogen | Synthetic peptide corresponding to amino acid residues near an internal region of ERK2 conjugated to KLH |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C (avoid freeze/thaw cycles) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
- Unconjugated
- DyLight 680
- DyLight 800
- FITC
- HRP
- Custom conjugation available upon request
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid freeze/thaw cycles. Centrifuge product if not completely clear after standing at room temperature. The product is stable for several weeks at 4°C as an undiluted liquid and should be diluted only prior to immediate use.
Reacts specifically with human ERK2. Purified from tissue culture supernatant by Protein A purification. Cross-reactivity is expected with human, mouse, and rat based on sequence identity of the peptide immunogen. This antibody does not react with the ERK1 isoform.
Target Information
MAPK1 (ERK2) is a serine/threonine kinase that acts as an important activator of p90 RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway. MAP kinases (extracellular signal-regulated kinases, ERKs) integrate multiple biochemical signals and are involved in cellular processes such as proliferation, differentiation, transcription regulation, and development. Activation requires phosphorylation by upstream kinases, after which ERK2 translocates to the nucleus to phosphorylate nuclear targets.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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