Thermo Fisher Scientific Ki-67 Monoclonal Antibody (SolA15), Brilliant Ultra Violet 661, eBioscience

Applications

Tested Application

• Flow Cytometry (Flow): 1.0 µg/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 532
• Alexa Fluor 660
• Alexa Fluor 700
• Alexa Fluor Plus 594
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395–805 Series
• Brilliant Violet 421–786 Series
• eFluor 450–660 Series
• FITC
• PE and PE Derivatives
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein present during all active phases of the cell cycle (G1, S, G2, mitosis) but absent in resting (G0) cells. Ki-67 localization changes from the nucleus during interphase to the chromosomes during mitosis. It serves as a marker for cell proliferation and is used to determine the growth fraction in tumor studies.

The SolA15 antibody also recognizes human, non-human primate, and canine Ki-67.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Tested using mouse splenocytes with the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00). Recommended use ≤1.0 µg/test in 100 µL final volume.

Note: Optimize antibody titration for best performance.

Fluorochrome Information:
Brilliant Ultra Violet™ 661 (BUV661) emits at 660 nm and is designed for cytometers with a 355 nm UV laser. Protect from light due to photo-induced oxidation sensitivity.

When using multiple Brilliant Ultra Violet™, Brilliant Violet™, or polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product #SB-4401-42) or Brilliant Stain Buffer™ (Product #00-4409-75) to reduce non-specific interactions.

Fixation:
Samples may be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark with minimal signal loss.

Excitation: 350 nm
Emission: 660 nm
Laser: Ultraviolet

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

Ki-67 is a nuclear protein expressed during late G1, S, G2, and M phases, but absent in G0. It associates with euchromatin and heterochromatin regions and shows cell cycle–dependent distribution. Ki-67 is widely used as a proliferation marker in cancer research, correlating with tumor grade and growth rate. It is also used as a neuronal marker of cell cycling and proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.