Thermo Fisher Scientific Armenian Hamster IgG Isotype Control (eBio299Arm), Brilliant Ultra Violet 661, eBioscience
Armenian hamster IgG 동형 대조 항체로서 유세포 분석에 사용됩니다. Brilliant Ultra Violet 661 형광체로 결합되어 UV 레이저 기반 장비에 적합합니다. 비특이적 결합을 최소화하며 음성 대조용으로 유용합니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific Armenian Hamster IgG Isotype Control (eBio299Arm), Brilliant Ultra Violet 661
Applications and Tested Dilution
- Flow Cytometry (Flow): Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Host/Isotype | Armenian hamster / IgG |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | eBio299Arm |
| Conjugate | Brilliant Ultra Violet™ 661 |
| Excitation/Emission Max | 349 / 659 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2929064 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488, 532, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395, 496, 563, 615, 737, 805
- Brilliant Violet 421, 480, 605, 650, 711, 786
- eFluor 450, 506, 615, 660
- FITC
- Functional Grade
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Custom conjugation available upon request
Product Specific Information
The eBio299Arm monoclonal antibody serves as an isotype control immunoglobulin.
- Reported for use in flow cytometric analysis.
- Tested on normal human peripheral blood and mouse splenocytes.
- Use at the same concentration as the experimental antibody.
Brilliant Ultra Violet™ 661 (BUV661) is a tandem dye emitting at 660 nm, suitable for cytometers equipped with a 355 nm ultraviolet laser. Ensure instrument compatibility.
When using multiple polymer dye-conjugated antibodies (Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer (00-4409-75) to minimize non-specific polymer interactions.
Light sensitivity: Protect vial and stained samples from light to prevent photo-induced oxidation.
Fixation: Samples can be stored in IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness or FRET efficiency.
Excitation: 350 nm
Emission: 660 nm
Laser: Ultraviolet Laser
BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.
Target Information
Isotype control antibodies help distinguish non-specific background signal from specific antibody signal.
They are most commonly used in flow cytometry, but also useful in ChIP, immunohistochemistry, and gel shift assays.
Controls should match the primary antibody’s species and isotype, and for directly labeled antibodies, use controls conjugated with the same fluorophore.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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