Thermo Fisher Scientific MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), Brilliant Ultra Violet 661, eBioscience

Thermo Fisher Scientific MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), Brilliant Ultra Violet 661, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry
• Tested Dilution: 0.25 µg/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488, 532, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 496, 737
• Brilliant Violet 421, 605, 650, 711, 786
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue, Red, Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Custom conjugation available

Product Specific Information

The M5/114.15.2 monoclonal antibody reacts with mouse MHC class II glycoproteins (I-A/I-E), detecting polymorphic determinants on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from certain H-2 haplotypes.
Reported for use in flow cytometric analysis and tested on mouse splenocytes (≤0.25 µg/test). Optimal performance requires empirical titration.

Brilliant Ultra Violet™ 661 (BUV661) emits at 660 nm and is designed for cytometers with UV (355 nm) lasers. Ensure instrument compatibility.

Buffer recommendation: Use Super Bright Complete Staining Buffer or Brilliant Stain Buffer to minimize non-specific polymer interactions.
Light sensitivity: Protect from light to prevent photo-induced oxidation.
Fixation: Samples may be stored in IC Fixation Buffer or 1-step Fix/Lyse Solution for up to 3 days at 4°C in the dark.
Excitation: 350 nm; Emission: 660 nm; Laser: Ultraviolet.

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company. Powered by Sirigen™.

Target Information

MHC class II molecules are transmembrane glycoproteins expressed on antigen-presenting cells such as macrophages, dendritic cells, and B cells. They bind exogenous antigens and present them to T cells.
HLA and MHC antibodies are crucial in immunopeptidomics for neoantigen identification via LC-MS/MS.

For Research Use Only. Not for use in diagnostic procedures.

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