Thermo Fisher Scientific Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Brilliant Ultra Violet 661, eBioscience
Mouse IgG1 kappa 동형 대조 항체로, Flow Cytometry용 Brilliant Ultra Violet 661 형광 표지. 실험 항체와 동일 조건에서 사용 가능. 자외선(355 nm) 레이저 장비에 적합하며, 비특이적 결합 최소화를 위해 Stain Buffer 사용 권장. 빛에 민감하므로 어둡게 보관.
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Applications
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | Assay-Dependent |
| Control (Ctrl) | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | P3.6.2.8.1 |
| Conjugate | Brilliant Ultra Violet™ 661 |
| Excitation / Emission Max | 349 / 659 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2925463 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 647, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395, 496, 563, 615, 737, 805
- Brilliant Violet 421, 480, 605, 650, 711, 786
- eFluor 450, 506, 570, 615, 660
- FITC
- Functional Grade
- Pacific Orange
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Request custom conjugation available
Product Specific Information
- Description: Monoclonal mouse IgG1 kappa immunoglobulin useful as an isotype control.
- Applications Reported: Suitable for flow cytometric analysis.
- Applications Tested: Tested on mouse splenocytes and normal human peripheral blood cells. Use at the same concentration as the experimental antibody.
- Fluorochrome Information: Brilliant Ultra Violet™ 661 (BUV661) emits at 660 nm and is designed for cytometers with an ultraviolet (355 nm) laser. Confirm your instrument compatibility.
- Buffer Recommendation: When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.
- Light Sensitivity: Protect from light due to photo-induced oxidation sensitivity.
- Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.
- Excitation / Emission / Laser: 350 nm / 660 nm / Ultraviolet Laser.
Trademark Notice:
BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.
Target Information
Mouse IgG1 is a subtype of immunoglobulin G antibody produced by B cells in response to an antigen. It is the most abundant IgG subtype in serum and plays a key role in adaptive immune responses, including opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity.
It is widely used in research and diagnostic applications such as ELISA, Western blotting, and immunohistochemistry, as well as in therapeutic research including immunotherapy and vaccine development.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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