Thermo Fisher Scientific TER-119 Monoclonal Antibody (TER-119), Brilliant Ultra Violet 661, eBioscience

Thermo Fisher Scientific TER-119 Monoclonal Antibody (TER-119), Brilliant Ultra Violet 661, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 1.0 µg/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395
• Brilliant UV 615
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 480
• eFluor 450
• eFluor 506
• FITC
• Functional Grade
• PE
• PE-Cyanine5
• PE-Cyanine5.5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780
• Request custom conjugation

Product Specific Information

The TER-119 monoclonal antibody reacts with mouse erythroid cells from early proerythroblast to mature erythrocyte stages. The TER-119 antigen is present in yolk sac, fetal and newborn liver, but not expressed by BFU-E and CFU-E cells. Erythroleukemia cell lines are negative for TER-119 even after DMSO stimulation. The antigen is associated with surface glycophorin A but is not a typical glycophorin.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse bone marrow cells at ≤1.0 µg/test (100 µL final volume). Cell number 10⁵–10⁸ cells/test. Optimize by titration.

Brilliant Ultra Violet™ 661 (BUV661) emits at 660 nm and is compatible with ultraviolet (355 nm) laser cytometers. Verify instrument compatibility before use.

Staining Panels:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to reduce non-specific interactions.

Light Sensitivity: Protect vial and samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C in dark.

Excitation: 350 nm
Emission: 660 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company. Powered by Sirigen™.

Target Information

TER-119 is a lineage marker for erythroid cells from early proerythroblast to mature erythrocyte stages in adult blood, spleen, and bone marrow. It is also expressed in yolk sac and fetal liver. Not expressed by BFU-e or CFU-e stage cells.

For Research Use Only. Not for use in diagnostic procedures.

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