Thermo Fisher Scientific TLE1 Monoclonal Antibody (OTI2D8), TrueMAB
Human TLE1 단백질을 인식하는 Mouse monoclonal antibody로, WB, IHC, ICC/IF, Flow Cytometry에 적합합니다. Lyophilized 형태이며, 고순도 Affinity chromatography로 정제되었습니다. 연구용으로 NF-kappa-B 및 Wnt 신호전달 관련 연구에 활용됩니다.
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Thermo Fisher Scientific TLE1 Monoclonal Antibody (OTI2D8), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2D8 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 180–460 of human TLE1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
TLE1 is a transcriptional corepressor that interacts with multiple transcription factors.
It inhibits NF-kappa-B-regulated gene expression and transcriptional activation mediated by FOXA2, CTNNB1, and TCF family members in Wnt signaling.
Full-length TLE family member effects may be modulated by association with dominant-negative AES.
It also functions as a coactivator for ESRRG.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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