Thermo Fisher Scientific ELK1 Recombinant Rabbit Monoclonal Antibody (SU32-05)
ELK1 단백질을 인식하는 재조합 토끼 단클론 항체로 높은 특이성과 민감도를 제공. Western blot, IHC, ICC/IF 등 다양한 응용에 적합. HEK293 세포에서 발현되며, lot 간 일관성과 동물유래 성분이 없는 포뮬레이션. 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse, Rat, Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SU32-05 |
| Immunogen | Synthetic peptide within Human ELK1 aa 378–428 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809402 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
ELK1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors. It is phosphorylated by MAP kinase pathways at its C-terminal S/T motifs, with Ser383 phosphorylation being critical for transcriptional activation.
ELK1 serves as a direct target of activated MAP kinase and acts as a nuclear target for the ras-raf-MAPK signaling cascade. Biochemical studies show that ELK1 phosphorylation correlates with MAP kinase activity, and interfering mutants of MAP kinase block ELK1 activation in vivo.
Alternatively spliced transcript variants encoding the same protein have been identified for ELK1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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