Thermo Fisher Scientific MICA/B Monoclonal Antibody (6D4), eBioscience

Applications and Tested Dilutions

Product Specifications

Available Formats

• Alexa Fluor 488
• Functional Grade
• PE
• Request custom conjugation

Product Specific Information

The 6D4 monoclonal antibody reacts with human MHC class I chain-related (MIC) proteins, MICA and MICB. These proteins share 83% amino acid similarity and structural homology with classical HLA molecules but do not bind β2-microglobulin or peptide typical of HLA class I.

MICA and MICB are expressed on endothelial cells, fibroblasts, gastric epithelium, and PHA-stimulated T cells. They act as ligands for NKG2D receptors found on NK cells, γδ T cells, and αβ CD8+ T cells. Human cytomegalovirus (HCMV) can inhibit MICB function to evade NK cell detection. Expression of MICA and MICB has been observed in various epithelial tumors, including breast, lung, ovary, prostate, colon, and kidney.

Applications Reported:

• Flow cytometry
• Immunoprecipitation
• Immunohistochemistry

For functional assays, use Functional Grade purified 6D4 (Product #16-5788).

Applications Tested:
Tested by flow cytometric analysis of HeLa cells. Recommended ≤0.5 µg/test in 100 µL final volume. Optimal titration is advised depending on cell number (10⁵–10⁸ cells/test).

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

MICA encodes a highly polymorphic MHC (HLA) class I chain-related gene A. The protein is expressed on the cell surface but does not associate with β2-microglobulin. MICA functions as a stress-induced antigen recognized by intestinal epithelial γδ T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.