Thermo Fisher Scientific RNF144B Monoclonal Antibody (OTI3D7), TrueMAB
RNF144B 단백질을 인식하는 Mouse monoclonal antibody (Clone OTI3D7). Western blot, ICC/IF, Flow cytometry에 적합. Lyophilized 형태로 제공되며, PBS buffer와 trehalose 포함. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3D7 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–256 of human RNF144B produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
E3 ubiquitin-protein ligase that accepts ubiquitin from E2 ubiquitin-conjugating enzymes (UBE2L3 and UBE2L6) and transfers it to substrates such as LCMT2, promoting their degradation.
Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. Overexpression reduces ubiquitin-dependent stability of BAX, leading to protection from cell death, though it is not inherently anti-apoptotic.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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