Thermo Fisher Scientific RNF144B Monoclonal Antibody (OTI12B6), TrueMAB
인간 RNF144B 단백질을 인식하는 Mouse IgG1 단클론 항체로 ICC/IF 및 Flow Cytometry에 적합. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도로 사용 가능. 연구용으로만 사용되며, E3 유비퀴틴 리가아제 기능 연구에 활용됨.
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Applications
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100
Flow Cytometry (Flow)
- Tested Dilution: 1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI12B6 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–256 of human RNF144B produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
RNF144B is an E3 ubiquitin-protein ligase that transfers ubiquitin from E2 enzymes (UBE2L3, UBE2L6) to substrates such as LCMT2, promoting their degradation. It induces apoptosis via a p53-dependent but caspase-independent mechanism. Overexpression reduces BAX stability, leading to protection against cell death, though RNF144B itself is not anti-apoptotic.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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