Thermo Fisher Scientific GRB2 Monoclonal Antibody (M331)
GRB2 단백질을 인식하는 Mouse monoclonal antibody (Clone M331). Western blot, ICC/IF, IP에 적합. Human, Mouse, Rat 시료 반응성. Protein A로 정제된 액상 항체로, -20°C에서 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:1,000 |
| Immunoprecipitation (IP) | 1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M331 |
| Immunogen | Clone (M331) was generated from a full length recombinant rat Grb2 protein. This sequence has high homology to human and mouse Grb2. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.125 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2942650 |
Product Specific Information
This antibody detects a 24 kDa* protein corresponding to the molecular mass of Grb2 on SDS-PAGE immunoblots of rat PC12.
Target Information
GRB2 protein contains one SH2 domain flanked on both sides by an SH3 domain. In response to EGF, GRB2 binds, through its SH3 domains, to the guanine nucleotide exchange factor Sos. The GRB2-Sos complex then binds the EGF receptor. These interactions play a critical role in the activation of the p21 ras signaling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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