Thermo Fisher Scientific JNK1 Monoclonal Antibody (M267)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100

-

Immunohistochemistry (Frozen) (IHC (F))

1:1,000

-

Immunocytochemistry (ICC/IF)

1:100

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

M267

Immunogen

Clone M267 was generated from a recombinant protein corresponding to amino acid residues in the C-terminal region of human JNK1. This sequence has high homology to rat and mouse JNK1, and has homology to similar regions in JNK2 and JNK3.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

~0.25 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2942651

Product Specific Information

This antibody detects a 46 kDa* protein corresponding to the apparent molecular mass of JNK1 on SDS-PAGE immunoblots of human A431 and HeLa, as well as rat PC12 cells.

Target Information

MAPK8 (JNK1) is activated by the presence of misfolded proteins in the endoplasmic reticulum. It is also a crucial mediator of insulin resistance and obesity. It belongs to the MAPK superfamily of stress-activated protein kinases. MAPKs are Serine-threonine protein kinases that are activated in response to a variety of extracellular stimuli, and mediate signal transduction from the cell surface to the nucleus. JNK is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. JNK pathways are activated by stress and inflammatory signals. JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1 and JNK2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.