Thermo Fisher Scientific EphA2 Monoclonal Antibody (M049)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

1:100

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

1:100

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

M049

Immunogen

Clone (M049) was generated from a recombinant protein that included the extracellular region of human EphA2 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2942646

Product Specific Information

Clone M049 mouse monoclonal antibody detects a 125kDa* protein on SDS-PAGE Native immunoblots of human NCI-H2052 and MDA-MB-231 carcinomas, as well as the EphA2 C-terminal 60 kDa fragment in human A431 and A549. The antibody detects native EphA2, as well as aldehyde-fixed EphA2. The antibody works for Native western blot, immunoprecipitation, immunocytochemistry, and ELISA capture.

Target Information

EPHA2 belongs to the ephrin receptor subfamily of the protein tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediated developmental events, particularly in the nervous system. EPHA2 is overexpressed in many types of human cancers. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.