Thermo Fisher Scientific EphA2 Monoclonal Antibody (M049)
EphA2 단백질을 인식하는 Mouse Monoclonal Antibody (Clone M049). Western blot, ICC/IF, ELISA, IP에 적합. Human 시료 반응성. Protein G 정제 및 PBS/BSA/glycerol buffer 구성. -20°C 보관, 연구용 전용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M049 |
| Immunogen | Clone (M049) was generated from a recombinant protein that included the extracellular region of human EphA2 protein. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2942646 |
Product Specific Information
Clone M049 mouse monoclonal antibody detects a 125 kDa protein on SDS-PAGE native immunoblots of human NCI-H2052 and MDA-MB-231 carcinomas, as well as the EphA2 C-terminal 60 kDa fragment in human A431 and A549.
The antibody detects native EphA2, as well as aldehyde-fixed EphA2.
It is suitable for native western blot, immunoprecipitation, immunocytochemistry, and ELISA capture.
Target Information
EPHA2 belongs to the ephrin receptor subfamily of the protein tyrosine kinase family.
EPH and EPH-related receptors are implicated in developmental processes, particularly in the nervous system.
EPHA2 is overexpressed in many types of human cancers.
The tyrosine kinase group regulates cell-cell interactions such as differentiation, adhesion, motility, and apoptosis.
Currently, about 90 TK genes are sequenced — 58 receptor protein TKs (e.g., EGFR, EPH, FGFR, PDGFR, TRK, VEGFR families) and 32 cytosolic TKs (e.g., ABL, FAK, JAK, SRC families).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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