Thermo Fisher Scientific GM130 Monoclonal Antibody (M342)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

-

Immunocytochemistry (ICC/IF)

1:50

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

M342

Immunogen

Clone M342 was generated from a recombinant protein corresponding to amino acid residues in the C-terminal region of human GM130. This sequence has high homology to similar regions in rat and mouse GM130.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2942649

Product Specific Information

This antibody detects a 130 kDa* protein corresponding to the apparent molecular mass of GM130 on SDS-PAGE immunoblots of mouse C2C12 cells. In immunocytochemistry, anti-GM130 specifically stains Golgi in mouse C2C12 cells.

Target Information

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.