Thermo Fisher Scientific PIST Recombinant Rabbit Monoclonal Antibody (ARC1831)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

ARC1831

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human PIST/GOPC (Q9HD26) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PIST,uniProtId:Q9HD26-1,ncbiNodeId:9606,antigenRange:1-100,antigenLength:462,antigenImageFileName:MA5-37819_PIST_Q9HD26-1_Rabbit.svg,antigenImageFileNamePDP:MA5-37819_PIST_Q9HD26-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.35 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2897742

Product Specific Information

Positive Samples: HepG2, U-87MG, Rat brain, Mouse lung

Immunogen sequence: MSAGGPCPAA AGGGPGGASC SVGAPGGVSM FRWLEVLEKE FDKAFVDVDL LLGEIDPDQA DITYEGRQKM TSLSSCFAQL CHKAQSVSQI NHKLEAQLVD

Target Information

PDZ domains contain approximately 90 amino acids and bind the extreme C terminus of proteins in a sequence-specific manner. PIST, a PDZ domain-containing Golgi protein, was discovered in a yeast two-hybrid system as a binding partner to Beclin-1, a Bcl-2-interacting protein homologous to the yeast autophagy gene apg6. Experiments with mutant PIST proteins lacking the PDZ domain showed that PIST interaction with Beclin-1 through its coiled-coil domain can modulate Beclin-1 activity and suggest that PIST interactions with other proteins through its PDZ domain may regulate the activity of PIST and Beclin-1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.