Thermo Fisher Scientific ATP6V1A Polyclonal Antibody
ATP6V1A 단백질을 인식하는 Thermo Fisher의 Rabbit Polyclonal 항체로, WB, IHC, IP 등 다양한 응용 가능. 인간, 마우스, 랫트 반응성 확인. 고순도 항원 친화 크로마토그래피 정제. 장기 보관 시 -20°C에서 안정적 유지.
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Thermo Fisher Scientific ATP6V1A Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunoprecipitation (IP) | 1:100–1:500 | - |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to amino acids 60–344 of Human ATP6V1A |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.57 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2546667 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2, mouse brain
- Predicted reactivity: Mouse (96%), Rat (95%), Xenopus laevis (90%), Pig (96%), Chicken (82%), Rhesus Monkey (99%), Bovine (98%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ATP6V1A encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme mediating acidification of eukaryotic intracellular organelles.
V-ATPase-dependent organelle acidification is essential for processes such as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation.
The enzyme consists of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain contains the ATP catalytic site and includes multiple subunits (A, B, G, C, D, E, F, H). The V0 domain comprises subunits a, c, c’, and d.
This protein represents one of two V1 domain A subunit isoforms and is expressed in all tissues. Transcript variants derived from alternative polyadenylation exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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