Thermo Fisher Scientific CD44/HCAM Std (Cancer Stem Cell Marker) Monoclonal Antibody (156-3C11)
CD44/HCAM 단클론 항체(156-3C11)는 암 줄기세포 마커로 사용되며, Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합합니다. 인간 및 비인간 영장류 반응성을 가지며, 단백질 A/G로 정제된 액상 형태로 제공됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 2–4 µg/mL |
| Flow Cytometry (Flow) | 2–4 µg/10⁶ cells |
| Functional Assay (Functional) | Assay-dependent |
| Immunomicroscopy (IM) | Assay-dependent |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 156-3C11 |
| Immunogen | Stimulated human leukocytes (CD44, UniProt ID: P16070-1, antigen range: 1–742) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
CD44 cell surface antigen is a 100 kDa type I transmembrane glycoprotein widely expressed on human leukocytes, white matter of the brain, and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues.
CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, adhesion, and migration. It participates in lymphocyte activation, recirculation, and homing. Expression may be upregulated in some carcinomas and has been linked to metastatic potential.
CD44 is expressed by hematopoietic and non-hematopoietic cells, epithelial tissues, and filopodia in cultured keratinocytes. Bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate CD44 expression in response to stimulation.
Alternative splicing produces multiple isoforms with distinct functions. CD44 dysfunction is associated with diseases such as superficial keratitis and lichen sclerosus, and it may also relate to tumor metastasis formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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