Thermo Fisher Scientific CD44/HCAM Std Monoclonal Antibody (SPM544)
CD44/HCAM 단클론 항체(SPM544)는 인간 및 비인간 영장류 반응성을 가지며, 단백질 A/G로 정제된 액상 형태입니다. WB, IHC, ICC/IF 등 다양한 응용에 적합하며, CD44 관련 세포 부착 및 이동 연구에 활용됩니다. 연구용 전용 제품입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 0.25–0.5 µg/mL |
| Immunocytochemistry (ICC/IF) | 2–4 µg/mL |
| Functional Assay | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SPM544 |
| Immunogen | Stimulated human leukocytes |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
CD44 cell surface antigen is a 100 kDa type I transmembrane glycoprotein widely expressed on human leukocytes, white matter of the brain, and some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 acts as a receptor for hyaluronic acid (HA) and plays roles in cell-cell interactions, adhesion, and migration.
It participates in lymphocyte activation, recirculation, and homing. CD44 expression may be up-regulated in certain carcinomas, potentially related to metastatic potential. It is expressed by hematopoietic and non-hematopoietic cells, epithelial tissues, and filopodia in cultured keratinocytes. Bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate CD44 in response to stimulation.
Transcripts for the CD44 gene undergo complex alternative splicing, generating many functionally distinct isoforms. Alternative splicing contributes to structural and functional diversity. Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus. CD44 may also be linked to tumor metastasis formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
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