Thermo Fisher Scientific PLK1 Monoclonal Antibody (OTI5C1), TrueMAB
PLK1 단백질을 인식하는 Mouse monoclonal 항체로, Western blot, IHC, Flow cytometry, Immunoprecipitation에 적합합니다. 세포 분열 및 암 관련 연구용으로 사용되며, 동결건조 형태로 제공되어 안정적인 보관이 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | 1:100 |
| Immunoprecipitation (IP) | 2 µg/500 µL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5C1 |
| Immunogen | Full-length protein expressed in 293T cells transfected with human PLK1 expression vector |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
PLK1 (Polo-like kinase 1) is a serine/threonine kinase involved in cell mitosis and is considered a marker of cell proliferation. It plays a critical role in carcinogenesis. PLK1 phosphorylates multiple substrates involved in centrosome function and spindle assembly, including BORA, CDC25C, CEP55, FOXM1, NEDD1, and WEE1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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