Thermo Fisher Scientific PLK1 Monoclonal Antibody (OTI1A2), TrueMAB
PLK1 단백질을 인식하는 Mouse IgG2b 모노클론 항체로, WB, IHC, Flow Cytometry, IP 등 다양한 응용에 적합합니다. 인간 PLK1 전체 길이 재조합 단백질을 면역원으로 사용하며, 고순도 친화 크로마토그래피 정제 및 무보존제 제형으로 제공됩니다.
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Thermo Fisher Scientific PLK1 Monoclonal Antibody (OTI1A2), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | Assay-dependent |
| Immunoprecipitation (IP) | 2 µg/500 µL |
Product Specifications
| Category | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1A2 |
| Immunogen | Full length human recombinant protein of human PLK1 produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
PLK1 (Polo-like kinase 1) is a serine/threonine kinase involved in cell mitosis and serves as a marker of cell proliferation. It plays a critical role in carcinogenesis and centrosome function.
PLK1 phosphorylates multiple proteins such as BORA, BUB1B, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5, FOXM1, KIF20A, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1, KIZ, PPP1R12A, PRC1, RACGAP1, SGOL1, STAG2, TEX14, TOPORS, TP73, TPT1, and WEE1.
Usage Notice
For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.
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