Thermo Fisher Scientific MDC Polyclonal Antibody, PeproTech
Human MDC(CCL22)에 특이적인 Rabbit Polyclonal Antibody로, Western blot, ELISA, IHC 등 다양한 응용에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공. 염증 반응 및 T-림프구 이동 연구에 적합.
- 카탈로그번호
- 500-P107-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific MDC Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | - |
| Immunohistochemistry (IHC) | – | 1 publication |
| ELISA | 0.5–2.0 µg/mL | 1 publication |
| Neutralization (Neu) | – | 1 publication |
| In vitro Assay (IV) | – | 1 publication |
| Immunostaining (IS) | – | 5 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human MDC (CCL22) (69 a.a.) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929222 |
Product Specific Information
AA Sequence of recombinant protein:
GPYGANMEDS VCCRDYVRYR LPLRVVKHFY WTSDSCPRPG VVLLTFRDKE ICADPRVPWV KMILNKLSQ
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human MDC (CCL22). The anti-Human MDC (CCL22)-specific antibody was purified by affinity chromatography employing an immobilized Human MDC (CCL22) matrix.
Sandwich ELISA:
To detect Human MDC (CCL22) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human MDC (CCL22) (500-P107Bt) as a detection antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human MDC (CCL22).
Western Blot:
To detect Human MDC (CCL22) by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human MDC (CCL22) is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Target Information
CCL22 may play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. It is chemotactic for monocytes, dendritic cells, and natural killer cells. CCL22 is a mild chemoattractant for primary activated T-lymphocytes and a potent chemoattractant for chronically activated T-lymphocytes, but has no chemotactic activity for neutrophils, eosinophils, or resting T-lymphocytes. It binds to CCR4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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