Thermo Fisher Scientific Bcl-2 (Apoptosis and Follicular Lymphoma Marker) Monoclonal Antibody (SPM530)
인간 Bcl-2 단백질을 표적하는 Thermo Fisher의 단클론 항체로, apoptosis 및 follicular lymphoma 연구용. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용 가능. Mouse/Rat 비반응성, 고순도 Protein A/G 정제, 연구용 전용.
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Thermo Fisher Scientific Bcl-2 (Apoptosis and Follicular Lymphoma Marker) Monoclonal Antibody (SPM530)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SPM530 |
| Immunogen | Synthetic peptide aa41–54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Does not react with Mouse or Rat.
Target Information
BCL-2 is a key regulator of apoptosis that can inhibit or promote cell death. Members of the BCL-2 family form dimers to modulate apoptosis. Bag1 forms heterodimers with BCL-2 to enhance its anti-apoptotic effect, while Bax and Bak promote cytochrome c release from mitochondria, initiating apoptosis. Overexpression or translocation of BCL-2 is associated with follicular lymphoma, where neoplastic germinal centers express high levels of BCL-2 alpha protein. Two transcript variants exist, differing at their C-terminal ends. BCL-2 overexpression is linked to cancers such as B-cell lymphoma and prostate cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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